|
|
Regulation of development of mouse parthenogenetic embryos
Jettmarová, Dominika ; Fulka, Josef (advisor) ; Kaňka, Jiří (referee)
The development of mouse (Mus musculus) haploid parthenogenetic embryos does not reach the same level as normal embryos. The aim of this diploma thesis was to find out whether haploid parthenogenetic embryos of mice differ in the nucleocytoplasmic ratio. The volume of the nucleus increases with ploidity. The nucleocytoplasmic ratios of haploid embryos do not significantly change between the two-cell and four-cell stage (p = 0.052), there is a significant difference (p < 0.001) for diploid and tetraploid embryos. Non-standard nucleocytoplasmic ratio could be related to the problematic development. Understanding the regulation of preimplantational development of parthenogenetic embryos will increase the efficiency of haploid embryonic stem cell derivation.
|
|
|
Regulation of development of mouse parthenogenetic embryos
Jettmarová, Dominika ; Fulka, Josef (advisor) ; Kaňka, Jiří (referee)
The development of mouse (Mus musculus) haploid parthenogenetic embryos does not reach the same level as normal embryos. The aim of this diploma thesis was to find out whether haploid parthenogenetic embryos of mice differ in the nucleocytoplasmic ratio. The volume of the nucleus increases with ploidity. The nucleocytoplasmic ratios of haploid embryos do not significantly change between the two-cell and four-cell stage (p = 0.052), there is a significant difference (p < 0.001) for diploid and tetraploid embryos. Non-standard nucleocytoplasmic ratio could be related to the problematic development. Understanding the regulation of preimplantational development of parthenogenetic embryos will increase the efficiency of haploid embryonic stem cell derivation.
|
|
|
Production and development of haploid mouse embryos
Jettmarová, Dominika ; Fulka, Josef (advisor) ; Petelák, Aleš (referee)
This bachelor thesis summarizes current knowledge of haploid embryos that are used for haploid embryonic stem cell derivation. The subject matter of this background research are production methods of haploid blastocysts of mice (Mus musculus) and the particularities of their development. Haploid blastocysts can be prepared parthenogenetically, gynogenetically or androgenetically. The development of haploid embryos is substantially different from the development of diploid embryos. The division of blastomeres is delayed by several hours and the success rate of development to blastocysts is low. The reason for the impaired development of haploid embryos is hypothesized to be improper activation and/or non-standard gene expression. The follow-up study that utilizes the Primo Vision monitoring system is described in the experimental part. Knowledge of this topic is crucial to raising the effectiveness of haploid blastocyst production and derivation of stable haploid embryonic stem cell lines for further biological and medical research. Powered by TCPDF (www.tcpdf.org)
|
|
|
Parthenogenetic lizards of the genus Darevskia as an evolutionary model
Abramjan, Andran ; Frynta, Daniel (advisor) ; Janko, Karel (referee)
Several parthenogenetic lineages occur within the lizards of the genus Darevskia (Sauria: Lacertidae) which are endemic to southern Transcaucasus. High level of heterozygosity, cause by thein hybrid origin, is one of the crucial aspects of thein evolutionary potential, as well as the asexual reproduction. Heterosis on one side is in the opposition to the outbreeding depression and genetic uniformity of the clones on the other side. Aim of this work is to evaluace if these aspects influence viability of parthenogenetic species and differ them from the sexual ones. We chose the amount of asymmetries as a measure of developmental instability, which we studied on three meristic characters. We also evaluated the pattern of asymmetries in lateral blue spots, which are of signaling importace in lacertid lizards. Our results suggest that there isn't significant difference between parthenogenetic and sexual species in developmental stability, but the sexual ones are more sensitive to population changes. Absence of males may have perhaps the greatest influence on coloration, resulting in loss of symmetry in the blue spots.
|
guest ::
